Next Generation Sequencing

Cokus et al. have just published a Nature paper on shotgun bisulphite sequencing of the Arabidopsis genome. The analysis was performed by combining bisulphite treatment of genomic DNA with ultra-high-throughput sequencing using the Illumina 1G Genome Analyser and Solexa sequencing technology. The authors named this approach BS-Seq, and state that it enabled them to “describe methylation on previously inaccessible components of the genome and analyse the DNA methylation sequence composition and distribution”