Dec 18
NGS platforms overview
Last update: November 25, 2009
We have elaborated on David Dooling’s original overview of some of the important metrics of the most prevalent High-Throughput Sequencing platforms.
| Roche | Illumina | ABI | ||||||||
| Technology: | 454 | Solexa | SOLiD | |||||||
| Platform: | Junior | GS 20 | FLX | Ti | GA | GA II | GA IIx | 1 | 2 | 3 |
| Reads: | 100 k | 500 k | 500 k | 1 M | 28 M | 100 M | 150 M | 40 M | 115 M | 320 M |
| Fragment | ||||||||||
| Read length: | 400 | 100 | 200 | 400 | 35 | 50 | 100 | 25 | 35 | 50 |
| Run time: | 12 hr | 6 hr | 7 hr | 9 hr | 3 d | 3 d | 4 d | 6 d | 5 d | 8 d |
| Images: | ? | 11 GB | 13 GB | 27 GB | 500 GB | 1.1 TB | 1.7 TB | 1.8 TB | 2.5 TB | 1.9 TB |
| PA Disk: | ? | 3 GB | 3 GB | 15 GB | 175 GB | 300 GB | 350 GB | 300 GB | 750 GB | 1200 GB |
| PA CPU: | ? | 10 hr | 140 hr | 220 hr | 100 hr | 70 hr | 100 hr | NA | NA | NA |
| SRA: | ? | 500 MB | 1 GB | 4 GB | 30 GB | 50 GB | 75 GB | 100 GB | 140 GB | 600 GB |
| Fragment yield | ||||||||||
| Gigabases / run | 0.035 | 0.05 | 0.1 | 0.5 | 1 | 5 | 15 | 1 | 4 | 16 |
| Megabases / hour | 2.92 | 8.3 | 14.3 | 55.6 | 13.9 | 69.4 | 156.3 | 6.9 | 33.3 | 83.3 |
| Gigabases / week | 0.5 | 1.4 | 2.4 | 9.3 | 2.3 | 11.7 | 26.3 | 1.2 | 5.6 | 14 |
| Paired-end | ||||||||||
| Read length: | 200 | 400 | 2×35 | 2×50 | 2×100 | 2×25 | 2×35 | 2×50 | ||
| Insert: | 3.5 kb | 3.5 kb | 200 b | 200 b | 200 b | 3 kb | 3 kb | 3 kb | ||
| Run time: | 7 hr | 9 hr | 6 d | 10 d | 10 d | 12 d | 10 d | 16 d | ||
| Images: | 13 GB | 30 GB | 1 TB | 2.2 TB | 3.4 TB | 3.6 TB | 5 TB | 3.8 TB | ||
| PA Disk: | 3 GB | 15 GB | 350 GB | 500 GB | 600 GB | 600 GB | 1.5 TB | 2.4 TB | ||
| PA CPU: | 140 hr | 220 | 160 hr | 120 hr | 170 hr | NA | NA | NA | ||
| SRA: | 1 GB | 4 GB | 60 GB | 100 GB | 150 GB | 200 GB | 280 GB | 1200 GB | ||
| Paired-end yield | ||||||||||
| Gigabases / run | 0.1 | 0.5 | 2 | 9 | 30 | 2 | 8 | 32 | ||
| Megabases / hour | 14.3 | 55.6 | 13.9 | 37.5 | 125 | 13.9 | 66.7 | 166.7 | ||
| Gigabases / week | 2.4 | 9.3 | 2.3 | 6.3 | 21 | 2.3 | 11.2 | 28 | ||
This overview is a work in progress and will be updated as new and relevant technologies emerge.
You can find the original table here. As the original compilation, this work is openly available through an Attribution-Share Alike 3.0 Creative Commons license.
December 24th, 2008 at 17:09
[...] Thanks to the beauty of Creative Commons, the folks over at CLC bio have posted a version of my next-generation sequencing informatics table that adds a new metric, Yield per hour: NGS Platform Overview. [...]
November 3rd, 2009 at 18:14
[...] working with genomics and high-throughput sequencing data analysis knows that you need serious number crunching capabilities to analyze the vast amounts [...]